半仿生酶法提取银杏总黄酮的工艺筛选及优化【摘要】 目的：筛选提取银杏叶中黄酮的半仿生酶法最佳提取工艺。
方法：以黄酮提取量为指标，先用乙醇回流法、半仿生法，半仿生酶法做实验。
得出半仿生酶法比半仿生提取效果好。
从半仿生酶法中筛选最佳提取工艺，通过单因素试验及响应面分析，考察提取液料液比、提取温度、酶量、提取时间对总黄酮提取量的影响。
得到的半仿生酶法的最佳工艺。
在最佳工艺下通过纯化酶使得提取率进一步提高。
【关键词】总黄酮、半仿生酶法、黄酮活性、工艺优化、分光光度法Screening and Optimization of the Extraction Technology of Total Flavonoids in ginkgo Leaves by semi-bionic-enzyme MethodABSTRACTOBJECTIVE:TO screen the method for extracting total flavonoids in ginkgo leaves and optimize extraction technology .METHODS:Using flavonoid extraction as an index, experiments were first conducted with ethanol reflux, semi-bionic, and semi-biomimetic enzymatic methods. It is concluded that the semi-bionic enzyme method is better than the semi-bionic extraction method. The optimum extraction process was selected from the semi-bionic enzymatic method. Through the single factor test and response surface analysis, the effects of extraction liquid-liquid ratio, extraction temperature, enzyme amount, and extraction time on the extraction amount of total flavonoids were investigated. The resulting semi-biomimetic enzymatic process is the best. Passing the purified enzyme under the optimum process further increases the extraction rate.KEYWORDSTotal flavonoids; Semi-bionic-enzyme; Flavonoids activity; Technology optimization; Spectrophotometry1、前言银杏叶被誉为植物活化石，起源于东亚国家，是银杏科植物银杏的干燥叶。
【1】秋季叶尚绿采收，及时干燥。
一般为人工栽培。
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